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AG Neuroproteomics/Connectomics

Study group

Prof. Dr. med. Oliver Schmitt
Dr. Peter Eipert
Dr. Michael Wille
Dipl.-Inf. Sebastian Schwanke
Antje Schümann
Christian Nitzsche

Neuroproteomics

The differential expression of proteins of cortical and sucortical regions are investigated by 2D-gelelectrophoresis and MS-identification. The analysis is performed over major points of postnatal (pp) development: 0-10 days pp, 14-21 days pp, 40 days pp and > 40 days pp. The differential expression of proteins from time step to time step should provide information of those proteins which are important for the differentiation of neuronal progenitor cells and the patterns of proteins of their exratracellular niches.

Connectomics

The connectome of the rat nervous system consists of connections between regions of the central and peripheral nervous system. Regions are considered at different levels of resolution down to particular populations of neurons and individual types of neurons. The connectome is characterized at different scales with regard to graph theoretical measures (neuroVIISAS, http://neuroviisas.med.uni-rostock.de/neuroviisas.html). The connections are availbale in a database (http://neuroviisas.med.uni-rostock.de/connectome/index.php) and we are continously developing new methods of visualization high dimensional data (visual analytics). The connectome is embeded in a neuroontology and fully linked with the all tract tracing reports of the rat nervous system. The connectome data can directly applied to computational neuroscience for population based simulation of the simulation engine NEST.

Publications (since 2010)

Schmitt O, Badurek S, Liu W, Wang Y, Rabiller G, Kanoke A, Eipert P, Liu  J (2017) Prediction of regional functional impairment following  experimental stroke via connectome analysis. Sci Rep, in press.

Wille M, Schümann A, Wree A, Kreutzer M, Glocker MO, Mutzbauer G, Schmitt O (2017) The differential proteome of the cerebral cortex of juvenile, adult and aged rats. J Proteom Bioinf, in press.

Mehlan J, Brosig H, Schmitt O, Mix E, Wree A, Hawlitschka A (2016) Intrastriatal injection of botulinum neurotoxin-A is not cytotoxic in rat brain - A histological and stereological analysis. Brain Res 1630: 18-24.

Claassen H, Schmitt O, Schulze M, Wree A (2016) Variations of brachial plexus with respect to concomitating aberrant arm arteries. Ann Anat 208: 40-48.

Schmitt O, Eipert P, Kettlitz R, Lessmann F, Wree A (2016) The connectome of the basal ganglia. Brain Struct Funct 221: 753-814.

Wille M, Schümann A, Wree A, Kreutzer M, Glocker MO, Mutzbauer G, Schmitt O (2015) The proteome profiles of the cerebellum of juvenile, adult and aged rats - An ontogenetic study. Int J Mol Sci 15: 21454-21485.

Wohlert D, Kröger J, Witt M, Schmitt O, Wree A, Czech-Damal N, Siebert U, Folkow L, Hanke FD (2015) A comparative morphometric analysis of three cranial nerves in two phocids: The hooded seal (CYSTOPHORA CRISTATA) and the harbor seal (PHOCA VITULINA). Anat Rec 299: 370-378.

Wille M, Schümann A, Kreutzer M, Glocker MO, Wree A, Schmitt O (2015) The proteome profiles of the olfactory bulb of juvenile, adult and aged rats - an ontogenetic study. Proteome Sci, 13: 8.

Prall F, Schmitt O, Schiffmann L (2015) Tumor regression in rectal cancer after intensified neoadjuvant chemoradiation: a morphometric and clinicopathological study. World Journal of Surgical Oncology 13:155.

Maass F, Petersen J, Hovakimyan M, Schmitt O, Witt M, Hawlitschka A, Lukas J, Rolfs A, Wree A (2015) Reduced cerebellar neurodegeneration after combined therapy with cyclodextrin/allopregnanolone and miglustat in NPC1 mutant mouse model (2015) J Neurosci Res 93: 433-442.

Weiss B, Haas S, Lessner G, Mikkat S, Kreutzer M, Glocker MO, Wree A, Schmitt O (2014) The proteome of the differentiating mesencephalic progenitor cell-line CSM14.1 in vitro. BioMed Res Int 2014: 1-13.

Antipova V, Hawlitschka A, Mix E, Schmitt O, Dräger D, Benecke R, Wree A (2013) Behavioural and structural effects of unilateral intrastriatal injections of botulinum neurotoxin A in the rat model of Parkinson's disease. J Neurosci Res, 91: 838-847.

Classen H, Schmitt O, Schulze M, Wree A (2013) Variations in the anatomy of hypothenar muscles and their impact on ulnar tunnel syndrome. Surg Rad Anat 35: 893-899.

Schmitt O, Usunoff KG, Lazarov NE, Itzev DE, Rolfs A, Wree A (2012) Orexinergic innervation of the extended amygdala and basal ganglia. Immunohistochemical study in the rat. Brain Struct Funct 217: 233-256.

Schmitt O, Eipert P (2012) neuroVIISAS: Approaching multiscale simulation of the rat connectome. Neuroinformatics 10: 243-267.

Schmitt O, Eipert P, Philipp K, Kettlitz R, Fuellen G, Wree A (2012) The intrinsic connectome of the rat amygdala. Front Neural Circuits 6:81.

Hovakimyana M, Müller J, Wree A, Rolfs A, Schmitt O (2012) Survival of transplanted human neural stem cell line (ReNcell VM) into the rat brain with and without immunosuppression. Ann Anat 194: 429-435.

Hawlitschka A, Antipova V, Schmitt O, Witt M, Benecke R, Mix E, Wree A (2012) Intracerebrally applied botulinum neurotoxin in experimental neuroscience. Curr Pharm Biotechnol, 14: 124-130.

Lange C, Mix E, Frahm J, Glass Ä, Mueller J, Schmitt O, Schmöle A-C, Klemm K, Ortinau S, Hübner R, Frech M, Wree A, Rolfs A (2011) Small molecule GSK-3 inhibitors increase neurogenesis of human neural progenitor cells. Neurosci Lett 488: 36-40.

Schmitt O, Birkholz H (2011) . Improvement in cytoarchitectonic mapping by combining electrodynamic modeling with local orientation in high-resolution images of the cerebral cortex. Micros Res Techn 74: 225-243.

Schmitt, O., Eipert, P., Wree, A., Schmitz, K.-P. (2011) Spike distributions of a population based hierarchical network of the rat amygdaloid complex. BMC Neurosci 12(Suppl 1): 1-2.

Röwer C, Ziems B, Radtke A, Schmitt O, Reimer T, Koy C, Thiesen H-J, Gerber B, Glocker MO (2011) Toponostics of Invasive Ductal Breast Carcinoma: Combination of Spatial Protein Expression Imaging and Quantitative Proteome Signature Analysis. Int J Clin Exp Path 4: 454-467

Wree A, Mix E, Hawlitschka A, Antipova V, Witt M, Schmitt O, Benecke R (2011) Intrastriatal botulinum toxin abolishes pathologic rotational behaviour and induces axonal varicosities in the 6-OHDA rat model of Parkinson's disease. Neurobiol Dis 41: 291-298.

Lazarov N, Usunoff K, Itzev D, Rolfs A, Wree A, Schmitt O (2011) Orexinergic innervation of the rat extended amygdala. Acta Morph Anthrop 17: 10-14.

Claassen H, Schmitt O, Wree A (2010) Superficial arm arteries revisited: Youg female patient with absent radialis pulse. Ann Anat 192: 151-155.

Hovakimyana M, Spasova M, Wree A, Schmitt O (2010) In vitro differentiation of the immortalized mesencephalic progenitor cell line CSM14.1 occurs independently from haematopoietic cytokines. Cell Molec Biol, 56: 1252-1260.

Schmitt O, Birkholz H (2010) A hybrid approach to quantify lamination of the cerebral cortex. Int J Nonlin Sci Sim 10: 1655-1661.

Lazarov NE, Usunoff KG, Schmitt O, Itzev DE, Rolfs A, Wree A (2010) Amygdalotrigeminal projection in the rat: An anterograde tracing study. Ann Anat 193:118-126.

Schmitt O, Lessner G, Haas SJ-P, Mikkat S, Kreutzer M, Wree A, Glocker MO (2010) The differential proteome of the striatum from hemiparkinsonian rats displays vivid structural remodeling processes. J Prot Res 9: 4671-4687.

Hildebrandt S, Schmidt A, Stoll A, Schmitt O, Köhling R, Wree A, Haas SJ-P, Pützer BM (2010) Targeting of neural stem cells in the hippocampus of adult rats by custom-made Ad vectors. Brain, Struct Funct 215:105-113.

Usunoff KG, Lazarov NE, Itzev DE, Schmitt O, Rolfs A, Wree A (2010) Projections from the central amygdaloid nucleus to the mesencephalic trigeminal nucleus in the rat. Acta morph anthrop, 15: 11-15.

Cooperations

• Prof. Dr. Michael O. Glocker, Proteome Center Rostock
• Prof. Dr. Jan Modersitzki, Institute of Mathematics and Image Computing, Lübeck

Contact person

Prof. Dr. med. Oliver Schmitt  
Hochschullehrer, Facharzt für Anatomie

Tel.: +49 (0)381 - 494 8408 
Fax. +49 (0)381 - 494 8402 
Raum: 2.14  
E-Mail: schmitt(at)med.uni-rostock.de