Dr. rer. hum. Sarah Joost

Kontakt:

Universitätsmedizin Rostock
Institut für Anatomie Rostock 
Dr. rer. hum. Sarah Joost  
Gertrudenstraße 9  

18057 Rostock

Tel.: +49 (0)381 - 494 8444
Raum 3.49   
E-Mail: sarah.joost{bei}med.uni-rostock.de

Link: AG ProMyelo

Forschungsschwerpunkte/Scientific profile

Morphology of the murine choroid plexus

The choroid plexus is located in the ventricular system of the brain and responsible for production of the cerebrospinal fluid (CSF). It is built of a highly vascularized stroma and the tight plexus epithelium, forming the blood-CSF-barrier. Besides its role for fluid homoeostasis of the brain, the choroid plexus is discussed as a site of immune surveillance. Furthermore, it probably is an access path for activated immune cells into the central nervous system under pathological conditions.

Detailed knowledge on the three-dimensional morphology of the choroid plexus, its attachment to the brain parenchyma, blood supply routes and its spatial relationship to different spaces of the CNS (ventricular space, subarachnoid space, brain parenchyma) are the prerequisite for elucidating the anatomical route of immune cell invasion via the choroid plexus. However, in mice, the most common experimental organism, these complex structures have not yet been described in sufficient detail.

We analyze the morphology of the ventricle system and the choroid plexus by micro CT scanning and three-dimensional reconstruction supplemented by histological processing of paraffin-embedded sections. Special emphasis is put on border structures that might interfere with migration routes of immune cells like basal membranes and glial borders (glia limitans).

Working on this project:

Theresa Greiner (AG Promyelo)

Kathrin Manzhula (AG Promyelo)

Dr. Jens Runge (AG Response)

 


The mysterious hind limb paralysis

In our colony of RAG1-deficient mice, a surprisingly high number of animals spontaneously developed hind limb paralysis. To date, such symptoms are not described in literature for this strain. Due to the high observed incidence, we decided to clarify the cause of the symptoms.

Spines and spleens were dissected and processed for histology. Dense infiltrates of uniformly shaped cells filled both the spleen and the bone marrow cavities of vertebral bones. Furthermore, these cells infiltrated into the spinal canal at multiple sites. These cell infiltrates are further characterized by means of histology, immunohistochemistry and flow cytometry. Additionally, the structural integrity of vertebral bones is analyzed by micro CT scanning.

Working on this project:

M. sc. Liu Feifei (AG Promyelo)

Dr. Anna Richter (Institute for Hematology and Oncology Rostock)

Dr. Jens Runge (AG Response)


Publikationen (Auswahl ab 2010)

  • Kaddatz H, Joost S, Nedelcu J, Chrzanowski U, Schmitz C, Gingele S, Gudi V, Stangel M, Zhan J, Santrau E, Greiner T, Frenz J, Müller-Hilke B, Müller M, Amor S, van der Valk P, Kipp M (2020) Oligodendrocyte degeneration recruits cytotoxic CD8+ lymphocytes into the central nervous system. Glia. 2021 Apr;69(4):925-942.
  • Rohr SO, Greiner T, Joost S, Amor S, van der Valk P, Schmitz C, Kipp M (2020) Aquaporin-4 Expression during Toxic and Autoimmune Demyelination. Cells. 2020 Sep 28;9(10):2187.
  • Zhan J, Mann T, Joost S, Behrangi N, Frank M, Kipp M (2020) The Cuprizone Model: Dos and Do Nots. Cells. 2020 Mar 31;9(4):843.
  • Zhan J, Yakimov V, Rühling S, Fischbach F, Nikolova E, Joost S, Kaddatz H, Greiner T, Frenz J, Holzmann C, Kipp M (2019) High Speed Ventral Plane Videography as a Convenient Tool to Quantify Motor Deficits during Pre-Clinical Experimental Autoimmune Encephalomyelitis. Cells. 2019 Nov 14;8(11).
  • Joost S, Mikkat S, Wille M, Schümann A, Schmitt O (2019) Membrane protein identification in rodent brain tissue samples and acute brain slices. Cells 2019. 8(5), 423.
  • Rabenstein M, Peter F, Joost S, Trilck M, Rolfs A, Frech MJ (2017): Decreased calcium flux in Niemann-Pick type C1 patient-specific iPSC-derived neurons due to higher amount of calcium-impermeable AMPA receptors. Mol Cell Neurosci. 2017 Sep; 83:27-36.
  • Joost S, Kobayashi K, Wree A, Haas SJP (2017): Optimisation of murine organotypic slice culture preparation for a novel sagittal-frontal co-culture system. J Neurosci Methods. 2017 Jun 15;285:49-57.
  • Pasold J, Engelmann R, Keller J, Joost S, Marshall RP, Frerich B, Müller-Hilke B (2013): High bone mass in the STR/ort mouse results from increased bone formation and impaired bone resorption and is associated with extramedullary hematopoiesis. J Bone Miner Metab. 2013 Jan;31(1):71-81.

Curriculum vitae

06/2009 Erwerb der allgemeinen Hochschulreife am Niedersächsischen Internatsgymnasium Bad Bederkesa
10/2009-12/2014 Studium der Medizinischen Biotechnologie an der Universität Rostock
10/2011-01/2013 Studentische Hilfskraft in Institut für Immunologie, Universität Rostock
08/2014-06/2015 Wissenschaftliche Hilfskraft im Albrecht-Kossel-Institut für Neuroregeneration, Universität Rostock
12/2014 Master of Science „Funktionelle Untersuchungen eines humanen neuronalen in vitro Modells des Morbus Niemann-Pick Typ C1 basierend auf differenzierten patientenspezifischen induzierten pluripotenten Stammzellen“ im Albrecht-Kossel-Institut für Neuroregeneration, Universität Rostock
seit 12/2015 Wissenschaftliche Mitarbeiterin am Institut für Anatomie, Universität Rostock